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Xinsheng Pharmaceutical Instrument Co., LtdTo Know More about Xinsheng Purification Technology
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Understanding HPLC: A Precision Instrument That "Queues and Sorts" Molecules
In the laboratory, the three letters HPLC are almost everywhere. Its full name is High Performance Liquid Chromatography. It sounds forbidding, but if you picture it as a highly automated "customs sorting system," things become much clearer...
General Procedures for Establishing Chromatographic Separation and Purification Methods
5 Core Steps of Chromatographic Method Development
Overview of the 5 Core Steps
1. Clarify separation targets and physicochemical properties of samples, and establish analytical methods for target purity detection.
2. Retention Screeni...
Reversed-Phase Chromatography Column Selection Guide
The core of chromatographic column selection lies in identifying property differences between analytes and selecting a column capable of amplifying such differences.
I. Principles for Reversed-Phase Chromatography Column Selection
Retenti...
Summary of Baseline Noise Issues
The common baseline noise problems we often encounter are: ① regular baseline fluctuations; ② irregular baseline fluctuations.
I.Regular noise
Reason 1: There are air bubbles in the pump or detector (with air bubbles in the pump, the...
Troubleshooting Low Column Efficiency: Five Key System Issues in Liquid Chromatography
I. Excessive Dead Volume
1. Mixer Dead Volume
(1) Gradient Elution (Significant Impact)
Excess mixer dead volume leads to a large gradient delay volume. The practical gradient reaches the analytical column with notable lag, accompan...
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